【Summary】

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principal types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

【Intended Use】

The Total Aflatoxins ELISA Test Kit is a competitive ELISA for the quantitative analysis of aflatoxin in grain, nuts, spices and feed.

【Use Principles】

The kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin antigen is precoated on the wells. The precoated antigen compete the aflatoxin antibody (antibody solution) with aflatoxin in the sample, anti-Aflatoxin antibody binds to the Aflatoxin-HRP enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standard and the Aflatoxin concentration of the samples is derived.

【Cross Reactivity】

AFB₁………………………………………………………100%

AFB₂………………………………………………………50%

AFG₁………………………………………………………40%

AFG₂………………………………………………………10%

AFM₁………………………………………………………20%

【Materials Required But Not Provided】

1. Devices:

---- Microwell plate Reader (450nm/630nm)

---- Oscillator / Vortex

---- Centrifuge

---- Balance (sensibility: 0.01g)

---- Rubber suction bulb

---- Funnel

---- Centrifuge tube: 7mL, 50mL

---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

---- Erlenmeyer flask:100mL

---- Quantitative analysis filter paper

2. Reagent:

---- Methanol (AR)

---- Deionized water

---- Concentrated sulfuric acid (18mol/L)

【Provided Materials and Reagent】

Component	96 wells
Antigen coated plate	12 × 8 wells
5 vials of Standard	1mL each
AFT Enzyme Conjugate	12mL
AFT Antibody Solution	12mL
Substrate	10mL*2
Concentrated Wash Buffer (10×)	40mL
Reagent Reservoir	1
Plate Cover Sheet	2
Package Insert	1
Testing Report	1

Note: The concentrations of the standards are 0μg/kg, 1.5μg/kg, 5μg/kg, 15μg/kg and 50μg/kg respectively. (the real concentration of the standards is 1/25 of the set concentrations)