The immunoaffinity column can simultaneously adsorb Aflatoxin (AFT B1, AFT B2, AFT G1, AFT G2, AFT M1, AFT M2), Zearalenone.

(ZEN) and its derivatives (ZAN, α-ZOL, β-ZOL, α-ZAL, β-ZAL), Deoxynivalenol (DON) and 15-Acetyldeoxynivalenol (15-ADON), Ochratoxin (OTA), T-2 toxin and HT-2 toxin, fumonisin (FB1, FB2,FB3), sterigmatocystin from the sample extract solution, thereby having a highly targeted purification effect on the more than 20 toxins of the seven major types, and the samples that have be purified after passing through the column can be used in LC-MS (LC-MS/MS) analysis.

1. Overview:

The basis of the measurement is the antigen-antibody reaction. More than 20 antibodies of seven main toxins are connected to the column concurrently. After the sample is extracted and filtered, it is slowly passed through the immunoaffinity column. The toxins bind to the corresponding antibodies in the column. The immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Elute the toxin with eluent and then inject into the analytical instrument for detection.

Order No. : HCM4210;

Product Properties: AFT B1/B2/G1/G2/M1/M2: 300, FB1/FB2/FB3: 5000, OTA: 100, ST:300, DON/15-ADON: 2000, ZEN: 1000, T-2/HT-2:1000;
Specification: 6mL,10T;
Samples: Grain,feed,etc.

2. Components of the kit:

Each kit contains 7 in 1 immunoaffinity columns and 1 instruction manual.

Necessary items not provided in the box:

Equipment

• LC-MS/MS
• Nitrogen gas evaporator apparatus
• Nitrogen gas tank and pressure regulator
• Air-pressure controller bracket
• Air pump
• Balance with 0.01g readability
• High-speed homogenizer (maximum speed > 10,000 r/ min) or shaker
• Grinder
• Sieving screen:2-mm
• Graduated cylinder: 100 mL/10 mL
• Funnel: 50 mL
• Syringe: 10 mL/20 mL
• Pipette: 1 mL and pipette tips
• Homogenization flask (or 250-mL conical flask with pestle)
• Sample bottles and tubes
• Rapid qualitative filter paper
• Microfiber filter paper (e.g. Whatman 934-AH)
• Column holder and syringe connector plug (for use with immunoaffinity columns)

3. Reagents

Methanol (CH3OH): Chromatography Grade
• Acetic acid (CH3COOH): Analytical Grade
• Acetonitrile (CH3CN): Analytical Grade
• Sodium chloride (NaCl): Analytical Grade
• TWEEN-20 (C58H114O26): Analytical Grade
• Sodium hydroxide (NaOH): Analytical Grade
• Potassium chloride (KCl): Analytical Grade
• Potassium dihydrogen phosphate (KH2PO4): Analytical Grade
• Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O): Analytical Grade
• Distilled or deionized water

4. Note:

Allow the immunoaffinity column to return to room temperature(22 to 25°C) before use.
The affinity column should be stored at 2 to 8°C, do not freeze.
Dot use any expired immunoaffinity column.
The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted accordingly.
The pH of the loading solution of the affinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.
Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent effects.

5. Column capacity:

Toxin name	Column capacity (ng)	Toxin name	Column capacity (ng)
AFT B1	300	ZEN	1000
AFT B2		ZAN
AFT G1		a-ZOL
AFT G2		P-ZOL
AFT M1		a-ZAL
AFT M2		P-ZAL
DON	2000	OTA	100
15-ADON		FB1	5000
T-2	1000	FB2
HT-2		FB3
ST	300

Aflatoxin, Zearalenone, Vomitoxin, Ochratoxin, T-2 toxins, sterigmatocystin and Fumonisin, as well as the derivatives of these toxins are toxic; and gloves should be worn when handling them.

The used container and toxin solution is best soaked with sodium hypochlorite solution (5%V/V) overnight.

Ensure the LC-MS/MS is clean and the tubing is primed appropriately for each run.