Background:

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.

Manufacturer	Meizheng
Trademark	ToxinFast®
Order No.	HEM0496
Analyte	Aflatoxins B1
Sample Type	Feed and feed material
Methodology	Indirect competitive enzyme-labeled immunoassay.
Packaging	96 Wells
Storage	The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf life	The validity for this kit is 12 months
Lead time	Shipped within 5-12 working days.
Cross Reactivity	AFB1……………………………………100% AFB2……………………………………38% AFG1……………………………………27% AFG2……………………………………4% AFM1………………..…………………20% AFM2……………………………………<1%
Calculate Result	Please use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples
Limit of detection	1.5µg/kg
Limit of quantitation	1.5µg/kg
Percent Recovery, %	100±20%
Precision (CV %):	Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

Component	QTY
Antigen coated plate	12×8 wells each
5 vials of Standard	1mL each
AFB1 Enzyme Conjugate	12mL
AFB1 Antibody Solution	12mL
Substrate	10mL*2
Concentrated Wash buffer (10×)	40mL
Package Insert	1
Plate Cover Sheet	2
Testing Report	1

Note: The concentrations of the standards are 0µg/kg, 1.5µg/kg, 5µg/kg, 15µg/kg and 50µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).

Materials Required But Not Provided

1. Devices:

---- Microwell plate Reader (450nm/630nm)

---- Oscillator/ Vortex

---- Thermostat incubator

---- Centrifuge (or quantitative filter)

---- Balance (sensibility: 0.01g)

---- Pipette: 50mL

---- Triangular flask (with stopper): 100mL

---- Centrifuge tube: 7mL

---- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL-300µL)

2. Reagent:

---- Methanol (AR)

---- Deionized water

---- Concentrated sulfuric acid (18 mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.